Human Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) ELISA Kit
The Human Inositol Polyphosphate 4-Phosphatase Type I (107kDa) (INPP4A) ELISA Kit is specifically designed for the quantitative detection of INPP4A levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and accuracy, ensuring precise and reliable results for a variety of research applications.INPP4A is an important enzyme involved in the regulation of inositol signaling pathways, playing a critical role in cellular processes such as cell growth, survival, and metabolism.
Dysregulation of INPP4A has been implicated in various diseases, including cancer, metabolic disorders, and neurological conditions, making it a valuable target for further investigation and therapeutic development.With its advanced technology and optimized protocols, the Human INPP4A ELISA Kit offers researchers a powerful tool for studying the role of INPP4A in health and disease, ultimately leading to a better understanding of its molecular mechanisms and potential clinical applications.
Product Name:
Human Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) ELISA Kit
Product Code:
HUDL01539
Size:
96 Assays
Target:
INPP4A
Synonyms:
INPP4, Inositol polyphosphate 4-phosphatase type I, Type I inositol 3,4-bisphosphate 4-phosphatase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
