The Human INHB (Inhibin B) ELISA Kit is specially designed for the precise measurement of inhibin B levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and dependable results, making it a valuable tool for various research purposes.Inhibin B is a key hormone that plays a critical role in regulating the reproductive system by inhibiting the production of follicle-stimulating hormone (FSH).
Imbalances in inhibin B levels are associated with infertility, polycystic ovary syndrome (PCOS), and other reproductive disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.With the Human INHB ELISA Kit, researchers can accurately measure inhibin B levels in human samples, advancing their understanding of reproductive health and paving the way for new diagnostic and treatment strategies.
Product Name:
Human INHB (Inhibin B) ELISA Kit
SKU:
HUES01537
Target:
Human INHB (Inhibin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INHB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INHB. You can calculate the concentration of Human INHB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
91-102
89-100
Average (%)
100
97
94
1:4
Range (%)
89-102
85-96
85-97
Average (%)
96
91
91
1:8
Range (%)
90-105
83-92
87-102
Average (%)
96
88
94
1:16
Range (%)
91-106
79-91
82-95
Average (%)
98
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.63
59.46
228.5
21.63
61.82
243.78
Standard deviation
1.39
3.03
7.24
1.17
3.42
9.92
C V (%)
5.88
5.1
3.17
5.41
5.53
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human INHB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INHB in samples. No significant cross-reactivity or interference between Human INHB and analogues was observed.
