The Human IL6R ELISA Kit is a reliable and precise tool for detecting IL6R levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it suitable for various research purposes.IL6R, also known as interleukin-6 receptor, plays a crucial role in the immune response and inflammatory processes. It is involved in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
Studying IL6R levels can provide valuable insights into the mechanisms underlying these diseases and aid in the development of targeted therapies.This ELISA kit is easy to use and includes all the necessary components for performing the assay. With its exceptional performance and versatility, the Human IL6R ELISA Kit is an essential tool for researchers studying the role of IL6R in health and disease.
Product Name:
Human IL-6R ELISA Kit
SKU:
HUES01459
Target:
Human IL-6R
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6R. You can calculate the concentration of Human IL-6R in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
92-106
89-101
Average (%)
95
98
95
1:4
Range (%)
92-108
80-94
84-96
Average (%)
100
86
90
1:8
Range (%)
86-98
82-96
87-102
Average (%)
92
89
94
1:16
Range (%)
92-105
86-100
88-103
Average (%)
99
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.05
260.8
888.96
95.01
238.49
860.92
Standard deviation
6.37
10.88
39.29
5.98
11.9
30.05
C V (%)
6.12
4.17
4.42
6.29
4.99
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-6R concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-6R in samples. No significant cross-reactivity or interference between Human IL-6R and analogues was observed.
