The Human IL-5 CLIA Kit is specifically developed for the precise measurement of Interleukin-5 (IL-5) levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for various research applications.IL-5 is a key cytokine that plays a crucial role in the immune response, particularly in regulating the growth and differentiation of eosinophils.
Dysregulation of IL-5 levels has been linked to various inflammatory conditions such as asthma, allergic rhinitis, and certain autoimmune disorders. Therefore, monitoring IL-5 levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies. By utilizing the Human IL-5 CLIA Kit, researchers can advance their understanding of the role of IL-5 in health and disease, ultimately leading to new diagnostic and therapeutic strategies.
Product Name:
Human IL-5 CLIA Kit
SKU:
HUES00165
Target:
Human IL-5
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-5. You can calculate the concentration of Human IL-5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
98-109
95-109
Average (%)
103
104
100
1:4
Range (%)
104-118
98-114
89-104
Average (%)
110
105
96
1:8
Range (%)
86-98
95-110
91-105
Average (%)
91
101
99
1:16
Range (%)
99-114
101-119
94-105
Average (%)
107
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.57
75.34
249.86
23.73
77.45
242.84
Standard deviation
2.27
7.03
15.39
2.03
7.57
24.28
C V (%)
8.88
9.33
6.16
8.55
9.77
10.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-5 in samples. No significant cross-reactivity or interference between Human IL-5 and analogues was observed.
