Interleukin-36 gamma (IL-36?) is a member of the interleukin 1 cytokine family that includes three closely related genes, IL-36?, β, and ?, formerly known as IL-1F6, F8, and F9 respectively. IL-36? has been detected in both neuronal and synovial tissue, whereas IL-36β and IL-36? are expressed in both cutaneous and mucosal epithelial cells, including the respiratory tract. IL-36β and IL-36? stimulate proliferation, maturation and/or cytokine expression by innate immune cells (such as keratinocytes and dendritic cells), and adaptive immune cells (neutrophils and T-cells) in both humans and mice. The activity of IL-36? is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). IL-36? plays an important role in communicating the cell death to surrounding cells.
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 5-20 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-36 gamma is produced by our E.coli expression system and the target gene encoding Ser18-Asp169 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.0 kDa
AP Mol Mass:
14-16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris,100mM Nacl,0.1mM EDTA,pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
