The Human IL-24 (Interleukin-24) ELISA Kit is a reliable and accurate tool for detecting IL-24 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it ideal for a wide range of research applications.IL-24 is a cytokine that plays a crucial role in immune responses and inflammation. It has been implicated in various diseases, including cancer, autoimmune disorders, and infectious diseases.
By measuring IL-24 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify new therapeutic targets.Overall, the Human IL-24 ELISA Kit is a valuable resource for researchers studying immune responses, inflammation, and disease pathology. Its high-quality components and performance ensure accurate and reliable results, making it an essential tool for advancing scientific knowledge in the field.
Product Name:
Human IL-24 ELISA Kit
SKU:
HUES03294
Target:
Human IL-24
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-24. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-24, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-24. You can calculate the concentration of Human IL-24 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
89-102
95-109
Average (%)
104
96
103
1:4
Range (%)
102-118
87-101
90-106
Average (%)
108
93
97
1:8
Range (%)
95-111
86-100
90-103
Average (%)
103
93
97
1:16
Range (%)
94-106
82-92
96-109
Average (%)
100
87
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
90-108
98
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.2
89.4
433.8
52.8
93.0
421.9
Standard deviation
3.3
4.9
18.2
2.7
4.2
20.3
C V (%)
6.71
5.48
4.2
5.11
4.52
4.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-24 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-24 in samples. No significant cross-reactivity or interference between Human IL-24 and analogues was observed.
