The Human IL-2 (Interleukin-2) ELISA Kit is specifically designed for the precise measurement of IL-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.IL-2 is a key cytokine that plays a crucial role in the regulation of immune responses, particularly in T cell activation and proliferation.
Dysregulation of IL-2 levels has been linked to autoimmune disorders, infectious diseases, and allergic reactions, highlighting its importance as a biomarker in immunological research and clinical studies.Overall, the Human IL-2 ELISA Kit offers researchers a reliable tool for studying the role of IL-2 in various physiological and pathological processes, aiding in the development of novel therapeutic strategies for immune-related disorders.
Product Name:
Human IL-2 ELISA Kit
SKU:
HUES01385
Target:
Human IL-2
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2. You can calculate the concentration of Human IL-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
96-111
92-105
Average (%)
103
103
98
1:4
Range (%)
96-111
88-98
99-109
Average (%)
104
95
104
1:8
Range (%)
102-113
91-97
96-111
Average (%)
105
94
104
1:16
Range (%)
89-101
88-104
94-107
Average (%)
95
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
93-104
100
Cell culture media (n=5)
93-102
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.64
58.38
217.66
23.12
56.78
226.32
Standard deviation
1.29
2.76
11.3
1.26
2.61
9.3
C V (%)
5.46
4.73
5.19
5.45
4.6
4.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-2 in samples. No significant cross-reactivity or interference between Human IL-2 and analogues was observed.
