The Human IL-2 (Interleukin-2) CLIA Kit is a powerful tool for the quantitative measurement of IL-2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-2 is a key cytokine that plays a critical role in the regulation of the immune response, specifically in the growth and differentiation of T cells. Dysregulation of IL-2 has been linked to various immune-related disorders, including autoimmune diseases and inflammatory conditions.
As such, measuring IL-2 levels can provide valuable insights into the immune system's functioning and help in the development of new therapeutic strategies.Whether you are studying immunology, oncology, or other areas of research, the Human IL-2 CLIA Kit from Assay Genie is an indispensable tool for investigating the role of IL-2 in health and disease. Trust in the accuracy and reliability of this kit to advance your research efforts effectively.
Product Name:
Human IL-2 CLIA Kit
SKU:
HUES00095
Target:
Human IL-2
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-2. You can calculate the concentration of Human IL-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
98-115
86-99
Average (%)
107
105
93
1:4
Range (%)
86-101
97-112
91-103
Average (%)
93
104
97
1:8
Range (%)
98-112
103-118
87-101
Average (%)
106
110
94
1:16
Range (%)
102-115
91-104
96-111
Average (%)
108
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
102
EDTA plasma (n=5)
97-112
104
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.59
79.59
328.3
38.61
75.31
318.54
Standard deviation
4.46
8.83
36.15
4.73
8.74
36.03
C V (%)
11.56
11.09
11.01
12.25
11.61
11.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-2 in samples. No significant cross-reactivity or interference between Human IL-2 and analogues was observed.
