Interleukin 1 receptor; type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A); interleukin beta (IL1B); and interleukin 1 receptor; type I (IL1R1/IL1RA); and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses; and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified; designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling; whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains; a single transmembrane region; and a short cytoplasmic domain; is expressed in a variety of cell types including B lymphocytes; neutrophils; monocytes; large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
Product Name:
Human IL1R2/CD121b Recombinant Protein (RPES1954)
Product Code:
RPES1954
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD121b,CDw121b,IL-1R-2,IL-1RT-2,IL-1RT2,IL1R2c,IL1RB,Interleukin-1 receptor type 2, IL-1R-2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, IL-1R-beta, Interleukin-1 receptor type I
Accession:
NP_004624.1
Sequence:
Met 1-Glu 343
Fusion tag:
C-His
Activity:
Measured by its ability to inhibit IL1?-dependent proliferation in D10.G4.1 mouse helper T cells.The ED50 for this effect is typically 5.5 µg/mL in the presence of 40 pg/mL of rhIL1?.2. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500pg/mL IL1b. The EC50 for this effect is typically 20-80ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1R2 (NP_004624.1) was expressed; with a fused C-terminal polyhistidine tag.
Purity:
> 98 % as determined by reducing SDS-PAGE.
Mol Mass:
39.3 kDa
AP Mol Mass:
50-55 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
