The Human IL1R1 (Interleukin-1 receptor type 1) ELISA Kit is specifically designed for the quantitative detection of IL1R1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.IL1R1 is a vital receptor involved in the inflammatory response, playing a key role in mediating the effects of interleukin-1.
Dysregulation of IL1R1 signaling has been associated with various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human IL1R1 ELISA Kit provides researchers with a reliable tool to accurately measure IL1R1 levels, contributing to a better understanding of the inflammatory response and its implications in disease pathology.
Product Name:
Human IL-1R1 ELISA Kit
SKU:
HUES02132
Target:
Human IL-1R1
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1R1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1R1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1R1. You can calculate the concentration of Human IL-1R1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
89-100
88-105
Average (%)
106
95
96
1:4
Range (%)
92-108
80-91
86-97
Average (%)
100
86
92
1:8
Range (%)
89-102
81-94
83-98
Average (%)
96
86
90
1:16
Range (%)
89-99
85-99
86-102
Average (%)
94
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.13
4.95
0.47
1.17
5.39
Standard deviation
0.03
0.06
0.21
0.03
0.06
0.27
C V (%)
6
5.31
4.24
6.38
5.13
5.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-1R1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1R1 in samples. No significant cross-reactivity or interference between Human IL-1R1 and analogues was observed.
