Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.
Product Name:
Human IL13RA2/CD213A2 Recombinant Protein (RPES3969)
Product Code:
RPES3969
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD213A2,CT19,IL-13R,IL13BP,IL13R
Accession:
NP_000631.1
Sequence:
Met 1-Leu342
Fusion tag:
C-His
Activity:
Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.02-0.08 µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human IL13R?2 (NP_000631.1) (Met1-Leu342) was expressed with the a polyhistidine tag at the C-terminus.
Purity:
> 85 % as determined by reducing SDS-PAGE.
Mol Mass:
38 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
