The Human IL12 p35 ELISA Kit is a powerful tool for measuring levels of IL12 p35 in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.IL12 p35 is a key cytokine that plays a vital role in regulating immune responses and promoting inflammation. Abnormal levels of IL12 p35 have been linked to various autoimmune diseases, infectious diseases, and inflammatory conditions.
By accurately measuring IL12 p35 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially develop targeted therapies.The Human IL12 p35 ELISA Kit from Assay Genie is an essential tool for researchers studying immune responses, inflammation, and related disorders. Its reliable performance and user-friendly design make it a valuable asset in any research laboratory.
Product Name:
Human IL-12 p35 ELISA Kit
SKU:
HUES02648
Target:
Human IL-12 p35
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12 p35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 p35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12 p35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-12 p35. You can calculate the concentration of Human IL-12 p35 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-103
97-115
Average (%)
92
95
105
1:4
Range (%)
91-107
82-95
86-97
Average (%)
98
87
91
1:8
Range (%)
86-98
86-96
85-95
Average (%)
93
91
90
1:16
Range (%)
90-104
85-100
85-99
Average (%)
95
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.29
57.49
248.98
24.48
53.32
233.14
Standard deviation
1.46
2.54
10.16
1.5
3.15
12.47
C V (%)
5.55
4.42
4.08
6.13
5.91
5.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-12 p35 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-12 p35 in samples. No significant cross-reactivity or interference between Human IL-12 p35 and analogues was observed.
