The Human IL-12 ELISA Kit is specifically designed for the precise measurement of Interleukin-12 (IL-12) levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.IL-12 is a key cytokine involved in regulating immune responses, particularly in the development of T-helper 1 (Th1) cells and enhancing the activity of natural killer cells and T cells.
Dysregulation of IL-12 has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By utilizing the Human IL-12 ELISA Kit, researchers can gain valuable insights into the role of IL-12 in immune regulation and disease pathogenesis, ultimately advancing our understanding of these complex processes.
Product Name:
Human IL-12 ELISA Kit
SKU:
HUES01424
Target:
Human IL-12
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-12. You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
98-112
89-100
Average (%)
96
104
94
1:4
Range (%)
100-115
88-101
98-113
Average (%)
106
93
104
1:8
Range (%)
101-112
84-94
92-107
Average (%)
106
89
99
1:16
Range (%)
97-113
85-96
96-108
Average (%)
105
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48
105.4
420.8
43.4
112.7
443.8
Standard deviation
3.2
4.7
17.3
2.8
5.2
23.5
C V (%)
6.67
4.46
4.11
6.45
4.61
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-12 in samples. No significant cross-reactivity or interference between Human IL-12 and analogues was observed.
