Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human IL beta/IL1B Recombinant Protein (RPES1518)
Product Code:
RPES1518
Size:
5µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
IL-1,IL-1 beta,IL-1B,IL1-BETA,IL1F2
Accession:
NP_000567.1
Sequence:
Ala 117-Ser 269
Fusion tag:
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 µg/mL (100?l/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.3. Measured by its binding ability in a functional ELISA.4. Immobilized human IL1B at 10 µg/mL (100 ?l/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 µg/mL.5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL1? (NP_000567.1) (Ala 117-Ser 269) was expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
