Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human IL-6/Interleukin-6 Recombinant Protein (RPES1238)
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 µg/ml (100 ?l/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 × 105 IU/µg.3. Measured by its binding ability in a functional ELISA. 2.Immobilized human IL6 at 10µg/mL (100?L/well) can bind human IL6R-His, the EC50 of human IL6R-His is 0.1-0.5 µg/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
20.3 kDa
AP Mol Mass:
20.3 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
