Human IL 32A Recombinant Protein (RPPB0661)
- SKU:
- RPPB0661
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- IL 32A
- Synonyms:
- NK4
- TAIF
- TAIFa
- TAIFb
- Source:
- Escherichia Coli
- Uniprot:
- P24001
Description
| Product Name: | Human IL 32A Recombinant Protein |
| Product Code: | RPPB0661 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL 32A |
| Synonyms: | NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL-32a, IL32a, IL32, Interleukin-32 alpha. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | IL-32 was lyophilized from a concentrated (1mg/ml) solution in water containing 50mM sodium Phosphate buffer pH=7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized IL-32 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL32 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL32 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K |
| Biological Activity: | Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha in the human THP-1 monocytic cell line. |
IL-32 is part of the cytokine family and contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. IL-32 expression is elevated after the activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 induces the production of TNF-a from macrophage cells. IL-32 pro-inflammatory pathway is activated in response to influenza A virus infection. Dysregulation of IL-32 in myelodysplastic syndrome and chronic myelomonocytic leukemia modulates apoptosis and impairs NK function. Induction of TNF, IL-1beta, and IL-6 by IL-32 is intervened by p38-MAPK. IL-32 induced monocyte-to-macrophage differentiation is mediated through nonapoptotic, caspase-3-dependent mechanisms. IL32 plays an important role in the pathogenesis of rheumatoid arthritis. IL-32 is involved in activation-induced cell death in T cells, through its intracellular actions. IL-32 is a cell-associated proinflammatory cytokine, which is particularly stimulated by mycobacteria through a caspase-1- and IL-18-dependent production of IFNgamma.IL-32 is associated with TNF-a, IL-1beta, and IL-18. IL32 is involved in human rheumatoid arthritis and is a novel target in autoimmune diseases.
Interleukin-32 human recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 131 amino acids and having a molecular mass of 14.9 kDa.
| UniProt Protein Function: | IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular space; membrane Molecular Function:protein binding; cytokine activity Biological Process: defense response; immune response; cell adhesion |
| NCBI Summary: | This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P24001 |
| NCBI GenInfo Identifier: | 61658632 |
| NCBI Gene ID: | 9235 |
| NCBI Accession: | NP_001012650.1 |
| UniProt Related Accession: | P24001 |
| Molecular Weight: | |
| NCBI Full Name: | interleukin-32 isoform B |
| NCBI Synonym Full Names: | interleukin 32 |
| NCBI Official Symbol: | IL32 |
| NCBI Official Synonym Symbols: | NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma |
| NCBI Protein Information: | interleukin-32 |
| UniProt Protein Name: | Interleukin-32 |
| UniProt Synonym Protein Names: | Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL32 |
| UniProt Entry Name: | IL32_HUMAN |
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