Human IL 3 Recombinant Protein (RPPB0497)
- SKU:
- RPPB0497
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- IL 3
- Synonyms:
- MCGF (Mast cell growth factor)
- Multi-CSF
- HCGF
- P-cell stimulation factor
- Source:
- Insect Cells
- Uniprot:
- P08700
Description
| Product Name: | Human IL 3 Recombinant Protein |
| Product Code: | RPPB0497 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL 3 |
| Synonyms: | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. |
| Source: | Insect Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5X PBS pH-7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg. |
IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Interleukin-3 Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine; Oncoprotein; Apoptosis Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-3 receptor binding; growth factor activity; cytokine activity Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of tyrosine phosphorylation of Stat5 protein; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication |
| NCBI Summary: | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08700 |
| NCBI GenInfo Identifier: | 124330 |
| NCBI Gene ID: | 3562 |
| NCBI Accession: | P08700.2 |
| UniProt Secondary Accession: | P08700,Q6GS87, |
| UniProt Related Accession: | P08700 |
| Molecular Weight: | 17,233 Da |
| NCBI Full Name: | Interleukin-3 |
| NCBI Synonym Full Names: | interleukin 3 |
| NCBI Official Symbol: | IL3 |
| NCBI Official Synonym Symbols: | IL-3; MCGF; MULTI-CSF |
| NCBI Protein Information: | interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; colony-stimulating factor, multiple; multilineage-colony-stimulating factor; multipotential colony-stimulating factor |
| UniProt Protein Name: | Interleukin-3 |
| UniProt Synonym Protein Names: | Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL3 |
| UniProt Entry Name: | IL3_HUMAN |
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