Human IL 17E Recombinant Protein (RPPB0603)
- SKU:
- RPPB0603
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- IL 17E
- Synonyms:
- IL-25
- IL-17E
- IL17E
- IL25
- Source:
- Escherichia Coli
- Uniprot:
- Q9H293
Description
| Product Name: | Human IL 17E Recombinant Protein |
| Product Code: | RPPB0603 |
| Size: | 25µg |
| Species: | Human |
| Target: | IL 17E |
| Synonyms: | IL-25, IL-17E, IL17E, IL25, Interleukin-25, Interleukin-17E. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution containing no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin Human IL25 in sterile 10mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin Human IL17E although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human IL-25 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG |
| Biological Activity: | The ED50 as determined by the dose-dependent production of IL-8 by Human PBMCs is 26-40ng/ml, corresponding to a specific activity of 3.8x104 units/mg. |
IL-25 also called IL-17E cytokine has a sequence similarity with IL17. IL-17E indluces NF-kappaB activation, and stimulates the production of IL-8. IL17E and IL17B are ligands for the cytokine receptor IL17BR. IL-25 is a proinflammatory cytokine favoring Th2-type immune response. The upregulation of costimulation-induced IL-17E receptors and release of cytokines and chemokines from IL-17E treated costimulated Th cells are differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. Blocking Iinterleukin-25 prevents airway hyperresponsiveness, a critical feature of clinical asthma. IL25 produced by innate effector eosinophils and basophils increase the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells. Over expression of IL-25 up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. IL-25 contributes to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts which supports the fact that IL-17E is an important factor in asthma pathophysiology. IL-17E operates by amplifying TH2 cell-mediated allergic airway inflammation but doesn’t induce allergic inflammation in vivo.
Interleukin-17E Human Recombinant produced in E.Coli is a non-glycosylated disulfide-linked homodimer containing 2x145 amino acids (with an N-terminal Methionine for each chain total aa for each chain 146) and having a total predicted molecular mass of 33.7kDa.The IL-25 Human is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular space Molecular Function:cytokine activity; interleukin-17E receptor binding Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q9H293 |
| NCBI GenInfo Identifier: | 20138730 |
| NCBI Gene ID: | 64806 |
| NCBI Accession: | Q9H293.1 |
| UniProt Secondary Accession: | Q9H293,Q2M3F0, Q8IZV3, Q8WXB0, |
| UniProt Related Accession: | Q9H293 |
| Molecular Weight: | 18,523 Da |
| NCBI Full Name: | Interleukin-25 |
| NCBI Synonym Full Names: | interleukin 25 |
| NCBI Official Symbol: | IL25 |
| NCBI Official Synonym Symbols: | IL17E |
| NCBI Protein Information: | interleukin-25; interleukin-17E |
| UniProt Protein Name: | Interleukin-25 |
| UniProt Synonym Protein Names: | Interleukin-17E; IL-17E |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL25 |
| UniProt Entry Name: | IL25_HUMAN |
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