Human IHH Recombinant Protein (RPPB0441)
- SKU:
- RPPB0441
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- IHH
- Synonyms:
- Indian hedgehog protein
- IHH
- HHG-2
- BDA1
- Source:
- Escherichia Coli
- Uniprot:
- Q14623
Description
| Product Name: | Human IHH Recombinant Protein |
| Product Code: | RPPB0441 |
| Size: | 25µg |
| Species: | Human |
| Target: | IHH |
| Synonyms: | Indian hedgehog protein, IHH, HHG-2, BDA1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized IHH in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG |
| Biological Activity: | Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 3.0-10.0?g/ml corresponding to a specific activity of 100-334units/mg. |
IHH belongs to the hedgehog family of secreted signaling molecules. Hedgehog proteins are vital regulators of various developmental processes including growth, patterning and morphogenesis. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). IHH has a specific role in bone growth and differentiation. In addition, IHH is involved in yolk sac vasculogenesis, having a central role in differentiation of epiblast cells into endothelial and red blood cells. IHH mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage in growth regions of the developing bone. IHH gene mutations cause the brachydactyly type A1 which is characterized by shortening or malformation of the phalanges and also the acrocapitofemoral dysplasia.
IHH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8kDa. The IHH is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q33-q35 Cellular Component: extracellular matrix; extracellular space; plasma membrane Molecular Function:peptidase activity; patched binding; calcium ion binding Biological Process: vitelline membrane formation; proteoglycan metabolic process; maternal process involved in pregnancy; cell maturation; positive regulation of collagen biosynthetic process; cell fate specification; proteolysis; embryonic pattern specification; response to estradiol stimulus; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; embryonic digestive tract morphogenesis; regulation of growth; pancreas development; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; negative regulation of T cell differentiation in the thymus; negative regulation of signal transduction; patterning of blood vessels; osteoblast differentiation; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; embryonic camera-type eye morphogenesis; positive regulation of alpha-beta T cell differentiation; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of alpha-beta T cell differentiation Disease: Brachydactyly, Type A1; Acrocapitofemoral Dysplasia |
| NCBI Summary: | This gene encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Feb 2010] |
| UniProt Code: | Q14623 |
| NCBI GenInfo Identifier: | 33112634 |
| NCBI Gene ID: | 3549 |
| NCBI Accession: | Q14623.4 |
| UniProt Secondary Accession: | Q14623,O43322, Q8N4B9, B9EGM5, |
| UniProt Related Accession: | Q14623 |
| Molecular Weight: | 45,251 Da |
| NCBI Full Name: | Indian hedgehog protein |
| NCBI Synonym Full Names: | indian hedgehog |
| NCBI Official Symbol: | IHH |
| NCBI Official Synonym Symbols: | BDA1; HHG2 |
| NCBI Protein Information: | indian hedgehog protein; HHG-2; Indian hedgehog homolog |
| UniProt Protein Name: | Indian hedgehog protein |
| UniProt Synonym Protein Names: | HHG-2Cleaved into the following 2 chains:Indian hedgehog protein N-product; Indian hedgehog protein C-product |
| Protein Family: | Indian hedgehog protein |
| UniProt Gene Name: | IHH |
| UniProt Entry Name: | IHH_HUMAN |
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