As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (Fc?R ) on phagocytic cells with high affinity.
Product Name:
Human IgG1 Fc Recombinant Protein (RPES0971)
Product Code:
RPES0971
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
g gamma-1 chain C region,IGHG1
Accession:
P01857
Sequence:
Asp104-Lys330
Fusion tag:
Activity:
Immobilized Human IgG1 Fc at 2µg/ml(100 ?l/well) can bind Human CD64-His(Cat: PKSH033655). The ED50 of Human IgG1 Fc is 47.99 ng/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
41.6 kDa
AP Mol Mass:
32 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
