The Human IgG (Immunoglobulin G) ELISA Kit is specifically designed for the quantitative detection of IgG levels in human serum and plasma samples. This kit provides accurate and reliable results with high sensitivity and specificity, making it an ideal tool for research in immunology, infectious diseases, and autoimmune disorders. Immunoglobulin G is the most abundant antibody in the human body and plays a crucial role in immune response by recognizing and binding to pathogens.
Monitoring IgG levels can provide valuable insights into the immune status of an individual, making this kit a valuable resource for studying immune system function and response to various pathogens. With its user-friendly protocol and efficient performance, the Human IgG ELISA Kit from Assay Genie is an essential tool for researchers and clinicians looking to investigate immune responses and develop new diagnostic and therapeutic strategies.
Product Name:
Human IgG (Immunoglobulin G) ELISA Kit
SKU:
HUES01439
Target:
Human IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgG. You can calculate the concentration of Human IgG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
91-106
96-111
Average (%)
98
97
104
1:4
Range (%)
100-114
89-103
97-108
Average (%)
107
94
103
1:8
Range (%)
94-108
81-91
90-103
Average (%)
102
87
97
1:16
Range (%)
98-111
84-95
96-114
Average (%)
103
89
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
87-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
12.5
33.6
5.1
11.5
36.5
Standard deviation
0.3
0.6
1.5
0.3
0.5
1.6
C V (%)
6.38
4.8
4.46
5.88
4.35
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IgG in samples. No significant cross-reactivity or interference between Human IgG and analogues was observed.
