Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
The Human IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) ELISA Kit is specifically designed for the accurate measurement of IGFBP-4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for various research applications.IGFBP-4 is a crucial protein that binds to insulin-like growth factors, regulating their activity and availability.
It plays a significant role in cell growth, differentiation, and apoptosis, making it a valuable biomarker for studying various physiological and pathological processes.With its reliable performance and user-friendly design, the Human IGFBP-4 ELISA Kit is an essential tool for researchers interested in investigating the role of IGFBP-4 in health and disease. Don't miss out on this valuable resource for your research needs.
Product Name:
Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
SKU:
HUES01656
Target:
Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-4. You can calculate the concentration of Human IGFBP-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-110
99-115
85-99
Average (%)
100
105
90
1:4
Range (%)
91-106
86-101
84-96
Average (%)
98
93
90
1:8
Range (%)
92-106
81-93
84-93
Average (%)
100
86
89
1:16
Range (%)
94-104
87-101
89-103
Average (%)
99
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
85-97
92
Cell culture media (n=5)
97-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.62
31.89
73.95
10.52
29.18
74.97
Standard deviation
0.6
1.79
3.21
0.59
1.47
3.63
C V (%)
5.65
5.61
4.34
5.61
5.04
4.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed.
