Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit
The Human IGFBP-3 (Insulin-like Growth Factor Binding Protein-3) ELISA Kit is a reliable tool for the precise measurement of IGFBP-3 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and consistent results for various research applications.IGFBP-3 is a critical regulator of insulin-like growth factor (IGF) signaling, modulating the bioavailability and activity of IGFs. It plays a crucial role in cell growth, differentiation, and apoptosis, making it a valuable biomarker in studies related to cancer, diabetes, and growth disorders.
By accurately quantifying IGFBP-3 levels, researchers can gain insights into its role in disease pathogenesis and potentially identify new therapeutic targets.Overall, the Human IGFBP-3 ELISA Kit offers researchers a dependable tool for investigating the complex interplay between IGFBP-3 and IGF signaling pathways, ultimately advancing our understanding of various physiological and pathological processes.
Product Name:
Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit
SKU:
HUES01373
Target:
Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-3. You can calculate the concentration of Human IGFBP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
89-100
84-97
Average (%)
100
95
90
1:4
Range (%)
86-100
82-93
90-102
Average (%)
93
89
95
1:8
Range (%)
88-100
81-92
82-96
Average (%)
94
86
88
1:16
Range (%)
87-103
85-99
81-95
Average (%)
94
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.56
6.62
20.79
2.65
7.06
19.42
Standard deviation
0.14
0.29
0.85
0.14
0.35
0.78
C V (%)
5.47
4.38
4.09
5.28
4.96
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-3 in samples. No significant cross-reactivity or interference between Human IGFBP-3 and analogues was observed.
