Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) CLIA Kit
The Human IGFBP-3 (Insulin-like Growth Factor Binding Protein-3) CLIA Kit is specially designed for the accurate quantification of IGFBP-3 levels in human serum and plasma samples. This kit offers excellent sensitivity and specificity, ensuring precise and reliable results for various research applications.IGFBP-3 is a key protein that plays a critical role in regulating the bioavailability of insulin-like growth factor (IGF). It functions as a carrier protein for IGF, influencing cell growth, differentiation, and survival.
Abnormal levels of IGFBP-3 have been linked to various diseases, including cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its high performance and efficiency, the Human IGFBP-3 CLIA Kit is an essential tool for researchers and healthcare professionals interested in investigating the role of IGFBP-3 in health and disease. Get reliable and reproducible results with this advanced assay kit from Assay Genie.
Product Name:
Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) CLIA Kit
SKU:
HUES00084
Target:
Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGFBP-3. You can calculate the concentration of Human IGFBP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
101-118
95-107
Average (%)
93
108
100
1:4
Range (%)
93-107
92-105
92-105
Average (%)
98
100
98
1:8
Range (%)
98-111
89-103
96-108
Average (%)
103
96
101
1:16
Range (%)
95-108
99-115
93-108
Average (%)
102
107
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.02
4.85
0.42
1.07
4.71
Standard deviation
0.06
0.1
0.52
0.05
0.11
0.37
C V (%)
13.04
9.8
10.72
11.9
10.28
7.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGFBP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-3 in samples. No significant cross-reactivity or interference between Human IGFBP-3 and analogues was observed.
