Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit
The Human IGFBP-1 (Insulin-Like Growth Factor Binding Protein-1) ELISA Kit is a reliable tool for the precise measurement of IGFBP-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IGFBP-1 is a key regulator of insulin-like growth factor (IGF) signaling, playing a crucial role in modulating cell growth, proliferation, and survival. Dysregulation of IGFBP-1 has been linked to various diseases such as diabetes, cancer, and metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
By using the Human IGFBP-1 ELISA Kit, researchers can gain valuable insights into the role of IGFBP-1 in disease pathogenesis and progression, ultimately advancing our understanding of the molecular mechanisms underlying these complex conditions. This kit is a valuable resource for studies aiming to elucidate the intricate interplay between IGF signaling and disease development.
Product Name:
Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit
SKU:
HUES01654
Target:
Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGFBP-1. You can calculate the concentration of Human IGFBP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
88-104
94-110
Average (%)
99
95
101
1:4
Range (%)
86-99
80-91
87-100
Average (%)
92
86
92
1:8
Range (%)
90-102
81-95
83-97
Average (%)
97
88
90
1:16
Range (%)
88-99
79-93
84-96
Average (%)
94
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
0.97
4.77
0.52
1.05
4.7
Standard deviation
0.03
0.05
0.2
0.03
0.06
0.15
C V (%)
5.77
5.15
4.19
5.77
5.71
3.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGFBP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGFBP-1 in samples. No significant cross-reactivity or interference between Human IGFBP-1 and analogues was observed.
