Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit
The Human IGF2BP3 (Insulin-like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit is a reliable tool for detecting levels of IGF2BP3 in human samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and consistent results for a variety of research applications.IGF2BP3 is a critical protein involved in regulating insulin-like growth factor 2 mRNA binding, which plays a role in cell growth and proliferation. Dysregulation of IGF2BP3 has been implicated in various diseases, including cancer and metabolic disorders, making it an important biomarker for studying these conditions and potentially developing targeted therapies.
With its high performance and ease of use, the Human IGF2BP3 ELISA Kit is a valuable tool for researchers studying the molecular mechanisms of cell growth and proliferation, as well as for those investigating potential therapeutic targets for diseases associated with IGF2BP3 dysregulation.
Product Name:
Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit
SKU:
HUES01650
Target:
Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF2BP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF2BP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF2BP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF2BP3. You can calculate the concentration of Human IGF2BP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
93-107
87-99
Average (%)
95
99
93
1:4
Range (%)
88-101
88-101
89-102
Average (%)
95
93
95
1:8
Range (%)
85-100
86-98
87-102
Average (%)
92
91
93
1:16
Range (%)
93-108
83-95
86-99
Average (%)
100
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
1.83
7.14
0.96
2.0
6.99
Standard deviation
0.05
0.1
0.29
0.06
0.09
0.33
C V (%)
4.9
5.46
4.06
6.25
4.5
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGF2BP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF2BP3 in samples. No significant cross-reactivity or interference between Human IGF2BP3 and analogues was observed.
