Human IGF1R (Insulin Like Growth Factor 1 Receptor) ELISA Kit
The Human IGF1R (Insulin-Like Growth Factor 1 Receptor) ELISA Kit is specifically designed for the accurate measurement of IGF1R levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The IGF1R protein, also known as the insulin-like growth factor 1 receptor, plays a key role in regulating cell growth, proliferation, and survival. Dysregulation of IGF1R signaling has been implicated in various diseases including cancer, diabetes, and metabolic disorders, making it a valuable biomarker for studying disease mechanisms and exploring potential therapeutic interventions.
With its advanced technology and precise detection capabilities, the Human IGF1R ELISA Kit is an essential tool for researchers working in the fields of oncology, endocrinology, and molecular biology. Get reliable and accurate results with this innovative kit for studying the role of IGF1R in human health and disease.
Product Name:
Human IGF1R (Insulin Like Growth Factor 1 Receptor) ELISA Kit
SKU:
HUES01638
Target:
Human IGF1R (Insulin Like Growth Factor 1 Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF1R. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF1R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF1R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF1R. You can calculate the concentration of Human IGF1R in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
83-96
92-106
Average (%)
101
90
98
1:4
Range (%)
85-101
87-100
89-101
Average (%)
92
92
94
1:8
Range (%)
89-102
85-95
82-92
Average (%)
96
90
87
1:16
Range (%)
87-98
81-92
86-99
Average (%)
93
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
101
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.62
9.14
1.03
2.42
9.3
Standard deviation
0.05
0.12
0.3
0.06
0.11
0.36
C V (%)
4.76
4.58
3.28
5.83
4.55
3.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IGF1R concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IGF1R in samples. No significant cross-reactivity or interference between Human IGF1R and analogues was observed.
