Human IGF1R (Insulin Like Growth Factor 1 Receptor) CLIA Kit
The Human IGF1R (Insulin-Like Growth Factor 1 Receptor) CLIA Kit is specifically designed for the precise measurement of IGF1R levels in human serum, plasma, and cell lysates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an excellent choice for various research applications. IGF1R is a key receptor involved in mediating the effects of insulin-like growth factor 1 (IGF1), which plays a crucial role in cell growth, differentiation, and survival. Dysregulation of the IGF1R signaling pathway has been implicated in various diseases, including cancer and diabetes, making it a valuable biomarker for studying these conditions and developing targeted therapies.
This CLIA kit offers a simple and efficient way to quantify IGF1R levels in biological samples, providing researchers with valuable insights into the role of this receptor in health and disease. Whether studying basic cell biology or exploring novel therapeutic strategies, the Human IGF1R CLIA Kit is a powerful tool for advancing biomedical research.
Product Name:
Human IGF1R (Insulin Like Growth Factor 1 Receptor) CLIA Kit
SKU:
HUES00307
Target:
Human IGF1R (Insulin Like Growth Factor 1 Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IGF1R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF1R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF1R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IGF1R. You can calculate the concentration of Human IGF1R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
91-109
101-115
Average (%)
109
99
106
1:4
Range (%)
102-118
85-97
90-101
Average (%)
108
92
96
1:8
Range (%)
101-118
90-105
96-106
Average (%)
108
98
101
1:16
Range (%)
100-118
90-102
91-107
Average (%)
108
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
95-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
248.98
785.66
2132.41
266.32
844.5
2150.23
Standard deviation
30.4
69.06
146.71
32.3
98.81
245.34
C V (%)
12.21
8.79
6.88
12.13
11.7
11.41
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IGF1R concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human IGF1R in samples. No significant cross-reactivity or interference between Human IGF1R and analogues was observed.
