Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases; which in turn phosphorylate several proteins; including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy; IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1; STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase; JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2; IFNAR2 is internalized; but; instead of being routed towards degradation as it is when complexed to IFNβ; it recycles back to the cell surface.
Product Name:
Human IFNAR2/IFNABR Recombinant Protein (RPES4442)
Product Code:
RPES4442
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
IFN-alpha-REC,IFN-R,IFNABR,IFNARB
Accession:
NP_997468.1
Sequence:
Met 1-Lys 243
Fusion tag:
C-His
Activity:
Measured by its ability to inhibit rh IFN? mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis.The EC50 for this effect is typically 1-4µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human IFNAR2 isoform a (NP_997468.1) (Met 1-Lys 243) was expressed; fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
26.2 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
