The Human IFN-Gamma (Interferon-Gamma) ELISA Kit is a powerful tool for accurate detection of IFN-Gamma levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.IFN-Gamma is a key cytokine involved in the regulation of immune responses, particularly in the activation of macrophages and the enhancement of T cell function. Its dysregulation is associated with various inflammatory conditions, autoimmune diseases, and even cancer.
Therefore, measuring IFN-Gamma levels can provide valuable insights into immune system function and potential disease mechanisms.With the Human IFN-Gamma ELISA Kit, researchers can quantitatively assess IFN-Gamma levels in biological samples with ease and precision, ultimately furthering our understanding of immune responses and potentially contributing to the development of new therapeutic approaches.
Product Name:
Human IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
HUES01394
Target:
Human IFN-gamma (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
92-105
89-105
Average (%)
105
98
96
1:4
Range (%)
98-110
86-99
91-105
Average (%)
103
92
97
1:8
Range (%)
96-110
82-98
98-114
Average (%)
102
89
104
1:16
Range (%)
97-111
85-99
95-109
Average (%)
105
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
101
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.9
98.0
447.2
55.8
100.1
476.7
Standard deviation
3.2
4.8
16.1
2.8
5.4
24.3
C V (%)
6.17
4.9
3.6
5.02
5.39
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.
