The Human IFN-Beta (Interferon-Beta) ELISA Kit is a highly reliable and sensitive tool for the precise measurement of IFN-Beta levels in human serum, plasma, and cell culture supernatants. With its high specificity, this kit ensures accurate and reproducible results, making it suitable for a wide range of research applications.IFN-Beta is a key player in the immune response, exerting anti-viral and anti-inflammatory effects by modulating immune cell activity.
Dysregulation of IFN-Beta signaling has been implicated in various autoimmune diseases, viral infections, and inflammatory disorders, highlighting its importance as a biomarker in disease research and potential therapeutic development.The Human IFN-Beta ELISA Kit from AssayGenie provides researchers with a valuable tool for studying the role of IFN-Beta in health and disease, offering reliable and precise quantification of this essential cytokine.
Product Name:
Human IFN- beta (Interferon Beta) ELISA Kit
SKU:
HUES01371
Target:
Human IFN- beta (Interferon Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-106
88-99
Average (%)
101
97
94
1:4
Range (%)
100-117
84-99
96-112
Average (%)
107
90
103
1:8
Range (%)
98-113
80-96
95-110
Average (%)
104
87
102
1:16
Range (%)
98-116
82-92
96-110
Average (%)
106
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.2
289.6
951.9
107.4
261.0
1001.0
Standard deviation
5.9
14.2
40.0
5.4
11.7
54.1
C V (%)
5.61
4.9
4.2
5.03
4.48
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed.
