Indoleamine 2,3-dioxygenase-like protein 1(IDO2) belongs to the indoleamine 2,3-dioxygenase family. IDO2 can be detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. It also expressed at low level in testis and thyroid but not expressed in the majority of human tumor samples. IDO2 catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. It involved in immune regulation. IDO1 and IDO2 are 2 distinct enzymes which catalyze the same reaction. IDO2 affinity for tryptophan is much lower than that of IDO1. 50 % of Caucasians harbor polymorphisms which abolish IDO2 enzymatic activity. IDO2 is expressed in human tumors in an inactive form: tryptophan degradation is entirely provided by IDO1 in these cells.IDO2 may play a role as a negative regulator of IDO1 by competing for heme-binding with IDO1. Lowefficiency IDO2 enzymes have been conserved throughout vertebrate evolution, whereas higher efficiencyIDO1 enzymes are dispensable in many lower vertebrate lineages. IDO1 may have arisen by geneduplication of a more ancient proto-IDO gene before the divergence of marsupial and eutherian(placental) mammals.
Product Name:
Human IDO2 Recombinant Protein (RPES2050)
Product Code:
RPES2050
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
INDOL1
Accession:
Q6ZQW0
Sequence:
Met14-Gly420
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human IDO2 is produced by our E.coli expression system and the target gene encoding Met14-Gly420 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
46.5 kDa
AP Mol Mass:
45 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris, 10% Glycerol,1mMEDTA,250mM NaCl,pH8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
