Human IDO1 Recombinant Protein (RPPB1845)
- SKU:
- RPPB1845
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- IDO1
- Synonyms:
- IDO
- IDO-1
- INDO
- Indoleamine 2
- Source:
- Escherichia Coli
- Uniprot:
- P14902
Description
| Product Name: | Human IDO1 Recombinant Protein |
| Product Code: | RPPB1845 |
| Size: | 10µg |
| Species: | Human |
| Target: | IDO1 |
| Synonyms: | IDO, IDO-1, INDO, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The IDO1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVK ALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG |
| Biological Activity: | Specific activity is > 300 pmol/min/ug, and is definedas the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan toN-formyl-Lkynurenine per minute at pH 6.5 at 25C. |
Indoleamine 2,3-Dioxygenase 1 (IDO1) catalyzes the primary and rate-limiting stage in tryptophan catabolism to N-formyl-kynurenine. IDO1 affects on various tryptophan substrates including D-tryptophan, and serotonin and is expressed in dendritic cells, monocytes, and macrophages. IDO1 takes part in a range of pathophysiological processes like neuropathology, antimicrobial and antitumor defense, immunoregulation, and antioxidant activity. IDO1 regulates T-cell behavior by its pericellular catabolization of the necessary amino acid tryptophan.
IDO1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 426 amino acids (1-403a.a) and having a molecular mass of 47.7kDa. IDO1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tryptophan; Cell cycle regulation; EC 1.13.11.52; Oxidoreductase Chromosomal Location of Human Ortholog: 8p12-p11 Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytosol Molecular Function:electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction |
| NCBI Summary: | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] |
| UniProt Code: | P14902 |
| NCBI GenInfo Identifier: | 123948 |
| NCBI Gene ID: | 3620 |
| NCBI Accession: | P14902.1 |
| UniProt Related Accession: | P14902 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | Indoleamine 2,3-dioxygenase 1 |
| NCBI Synonym Full Names: | indoleamine 2,3-dioxygenase 1 |
| NCBI Official Symbol: | IDO1 |
| NCBI Official Synonym Symbols: | IDO; INDO; IDO-1 |
| NCBI Protein Information: | indoleamine 2,3-dioxygenase 1 |
| UniProt Protein Name: | Indoleamine 2,3-dioxygenase 1 |
| UniProt Synonym Protein Names: | Indoleamine-pyrrole 2,3-dioxygenase |
| Protein Family: | L-isoleucine-4-hydroxylase |
| UniProt Gene Name: | IDO1 |
| UniProt Entry Name: | I23O1_HUMAN |
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