Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF ? and β-endorphin.
Product Name:
Human IDE/Insulysin Recombinant Protein (RPES1543)
Product Code:
RPES1543
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Insulin-Degrading Enzyme, Abeta-Degrading Protease, Insulin Protease, Insulinase, Insulysin, IDE
Accession:
P14735
Sequence:
Met42-Leu1019
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Insulin-Degrading Enzyme is produced by our Mammalian expression system and the target gene encoding Met42-Leu1019 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
114.3 kDa
AP Mol Mass:
120 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,0.05%Brij35,10%Glycerol,pH7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
