The Human IDE (Insulin-Degrading Enzyme) ELISA Kit is a reliable and sensitive assay designed for the quantitative detection of IDE levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it perfect for various research applications.IDE is a key enzyme involved in the regulation of insulin and amyloid beta degradation, playing a critical role in glucose metabolism and Alzheimer's disease pathology.
Its dysregulation has been implicated in diabetes and neurodegenerative disorders, highlighting its importance as a potential therapeutic target and biomarker for disease monitoring.With its high specificity and sensitivity, the Human IDE ELISA Kit offers a valuable tool for researchers studying IDE function, disease mechanisms, and potential therapeutic interventions. Get reliable and precise results with this advanced assay kit for IDE detection.
Product Name:
Human IDE (Insulin Degrading Enzyme) ELISA Kit
SKU:
HUES01641
Target:
Human IDE (Insulin Degrading Enzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
93-105
95-108
Average (%)
97
99
101
1:4
Range (%)
92-102
84-98
86-96
Average (%)
97
91
91
1:8
Range (%)
86-102
81-94
85-97
Average (%)
93
87
90
1:16
Range (%)
88-102
82-96
85-97
Average (%)
94
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.13
350.08
1527.78
228.03
347.06
1487.36
Standard deviation
14.45
15.51
78.53
11.56
18.74
56.82
C V (%)
6.75
4.43
5.14
5.07
5.4
3.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IDE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IDE in samples. No significant cross-reactivity or interference between Human IDE and analogues was observed.
