Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human ICOS Ligand/ICOSL Recombinant Protein (RPES1068)
Product Code:
RPES1068
Size:
100µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
ICOS Ligand, B7 Homolog 2, B7-H2, B7-Like Protein Gl50, B7-Related Protein 1, B7RP-1, CD275, ICOSLG, B7H2, B7RP1, ICOSL, KIAA0653
Accession:
O75144-1
Sequence:
Met 1-Ser258
Fusion tag:
C-Fc
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human ICOSLG (O75144-1) (Met1-Ser258) was expressed with the Fc region of human IgG1 at the C-terminus.
Purity:
(96.1+2.0) % as determined by reducing SDS-PAGE.
Mol Mass:
53.7 kDa
AP Mol Mass:
66-76 and 33 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
