Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
The Human I-TAC (Interferon-Inducible T Cell Alpha Chemoattractant) ELISA Kit is specifically designed for the precise detection of I-TAC levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it well-suited for various research applications.I-TAC is a key chemokine involved in immune response regulation, specifically in attracting and activating T cells. It plays a crucial role in inflammatory processes and immune cell recruitment to sites of infection or injury.
The measurement of I-TAC levels is essential for studying immune responses, inflammatory diseases, and potential therapeutic interventions.Overall, the Human I-TAC ELISA Kit provides researchers with a valuable tool for investigating the role of I-TAC in immune regulation and inflammatory processes, ultimately contributing to advancements in the understanding and treatment of various immune-related conditions.
Product Name:
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
SKU:
HUES01338
Target:
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human I-TAC/CXCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human I-TAC/CXCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human I-TAC/CXCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human I-TAC/CXCL11. You can calculate the concentration of Human I-TAC/CXCL11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
94-108
91-103
Average (%)
95
99
97
1:4
Range (%)
86-101
79-93
88-101
Average (%)
92
85
93
1:8
Range (%)
88-99
85-100
88-104
Average (%)
93
92
95
1:16
Range (%)
88-102
84-95
82-97
Average (%)
96
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
100
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.69
629.97
1461.83
202.91
631.91
1468.74
Standard deviation
13.1
30.36
56.87
10.84
36.97
54.93
C V (%)
6.98
4.82
3.89
5.34
5.85
3.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human I-TAC/CXCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human I-TAC/CXCL11 in samples. No significant cross-reactivity or interference between Human I-TAC/CXCL11 and analogues was observed.
