Human HTRA2 Recombinant Protein (RPPB1837)

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SKU:
RPPB1837
Product type:
Recombinant Protein
Size:
50ug
Species:
Human
Target:
HTRA2
Synonyms:
Serine protease HTRA2 mitochondrial
Synonyms:
EC 3421108
Synonyms:
High temperature requirement protein A2
Synonyms:
HtrA2
Source:
Escherichia Coli
Uniprot:
O43464
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Description

Product Name:Human HTRA2 Recombinant Protein
Product Code:RPPB1837
Size:50µg
Species:Human
Target:HTRA2
Synonyms:Serine protease HTRA2 mitochondrial, EC 3.4.21.108, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine proteinase OMI, Serine protease 25, OMI, PARK13, PRSS25.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The protein (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 50mM NaCl, 1mM DTT, and 20% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH

HtrA2 also called Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.

HtrA2 Human Recombinant amino acids 134-458 His-Tag fusion protein produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 36kDa.The HtrA2 is purified by proprietary chromatographic techniques.

UniProt Protein Function:HTRA2: a serine proteinase that normally resides in mitochondrial membranes. It exists in two populations in mitochondria: as an unprocessed form probably attached to the inner mitochondrial membrane through a N-terminal transmembrane domain, and as a processed form containing a reaper motif at its N-terminus. Following apoptotic stimuli it is autoproteolytically activated and released into the cytosol, where it promotes programmed cell death in caspase-dependent and -independent manners. The amino-terminal reaper motif binds to the IAP (inhibitor of apoptosis) proteins cIAP1, cIAP2, and and XIAP, disrupting IAP-caspase complexes in a manner similar to Smac/DIABLO. Phosphorylation by the protein kinase Akt attenuates its serine protease and pro-apoptotic activities. The PDZ domain mediates interaction with Mxi2, an alternatively spliced form of the p38 stress-activated kinase. In contrast to its pro-apoptotic effects, targeted deletion in mice indicates that it is involved in protection against cell stress in striatial neurons. Defects in HTRA2 are the cause of Parkinson disease 13 (PARK13). Four alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Protease; Mitochondrial; Membrane protein, integral; EC 3.4.21.108

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: chromatin; cytoskeleton; cytosol; endoplasmic reticulum membrane; internal side of plasma membrane; membrane; mitochondrial intermembrane space; mitochondrion; nucleus

Molecular Function:peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: cellular protein catabolic process; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of cell cycle; positive regulation of apoptosis; positive regulation of caspase activity; protein autoprocessing; proteolysis

Disease: Parkinson Disease 13, Autosomal Dominant, Susceptibility To

NCBI Summary:This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Code:O43464
NCBI GenInfo Identifier:17376879
NCBI Gene ID:27429
NCBI Accession:O43464.2
UniProt Secondary Accession:O43464,Q9HBZ4, Q9P0Y3, Q9P0Y4,
UniProt Related Accession:O43464
Molecular Weight:39,914 Da
NCBI Full Name:Serine protease HTRA2, mitochondrial
NCBI Synonym Full Names:HtrA serine peptidase 2
NCBI Official Symbol:HTRA2  
NCBI Official Synonym Symbols:OMI; PARK13; PRSS25  
NCBI Protein Information:serine protease HTRA2, mitochondrial
UniProt Protein Name:Serine protease HTRA2, mitochondrial
UniProt Synonym Protein Names:High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI
Protein Family:Serine protease
UniProt Gene Name:HTRA2  
UniProt Entry Name:HTRA2_HUMAN
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