The Human HTRA1 (HTRA Serine Peptidase 1) ELISA Kit is a powerful tool for detecting levels of HTRA1 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal choice for various research applications.HTRA1 is a serine protease involved in various biological processes, including protein degradation and cell signaling. Dysregulation of HTRA1 has been associated with multiple diseases, such as Alzheimer's disease, arthritis, and cancer, highlighting its importance as a potential biomarker for disease diagnosis and therapy development.
With the Human HTRA1 ELISA Kit, researchers can gain valuable insights into the role of HTRA1 in disease pathogenesis and progression, opening up new avenues for research and therapeutic interventions. Trust in the reliability and precision of this kit to advance your studies in the field of HTRA1 biology.
Product Name:
Human HTRA1 (HtrA Serine Peptidase 1) ELISA Kit
SKU:
HUES01636
Target:
Human HTRA1 (HtrA Serine Peptidase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HTRA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HTRA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HTRA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HTRA1. You can calculate the concentration of Human HTRA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
91-106
85-99
Average (%)
102
97
92
1:4
Range (%)
89-106
86-98
87-99
Average (%)
97
92
93
1:8
Range (%)
92-105
79-93
84-97
Average (%)
100
85
89
1:16
Range (%)
89-103
79-92
87-102
Average (%)
94
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.44
7.64
0.92
2.3
6.99
Standard deviation
0.05
0.12
0.24
0.05
0.11
0.26
C V (%)
5.43
4.92
3.14
5.43
4.78
3.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HTRA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HTRA1 in samples. No significant cross-reactivity or interference between Human HTRA1 and analogues was observed.
