Human HSPG (Heparan Sulfate Proteoglycan) ELISA Kit
The Human HSPG (Heparan Sulfate Proteoglycan) ELISA Kit is specifically designed for the quantitative detection of HSPG levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and consistent results for various research applications.HSPG is a key component of the extracellular matrix and cell surface, playing a critical role in cell adhesion, signaling, and growth factor binding. Dysregulation of HSPG expression has been linked to various diseases like cancer, inflammatory disorders, and neurological diseases, making it an important biomarker for studying disease progression and potential therapeutic interventions.
With the Human HSPG ELISA Kit, researchers can easily measure HSPG levels in human samples, facilitating their studies on HSPG's role in disease pathogenesis and potential therapeutic targeting. Trust in the reliability and precision of this ELISA kit for your research needs.
Product Name:
Human HSPG (Heparan Sulfate Proteoglycan) ELISA Kit
SKU:
HUES01634
Target:
Human HSPG (Heparan Sulfate Proteoglycan)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSPG. You can calculate the concentration of Human HSPG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
95-110
90-101
Average (%)
99
103
95
1:4
Range (%)
88-102
83-95
88-102
Average (%)
94
90
93
1:8
Range (%)
89-100
86-101
82-94
Average (%)
95
92
89
1:16
Range (%)
92-105
86-97
82-93
Average (%)
99
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
3.17
6.99
0.98
2.86
6.74
Standard deviation
0.06
0.15
0.24
0.06
0.15
0.24
C V (%)
6.38
4.73
3.43
6.12
5.24
3.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HSPG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSPG in samples. No significant cross-reactivity or interference between Human HSPG and analogues was observed.
