The Human Hsp-90 (Heat Shock Protein 90) ELISA Kit is designed to accurately measure levels of Hsp-90 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Hsp-90 is a key molecular chaperone that plays a critical role in protein folding, stability, and function. It is involved in various cellular processes, including cell cycle regulation, signal transduction, and stress responses. Dysregulation of Hsp-90 has been associated with cancer, neurodegenerative diseases, and other disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
With the Human Hsp-90 ELISA Kit, researchers can accurately quantify Hsp-90 levels in biological samples, allowing for better understanding of its role in health and disease. This kit is easy to use, reliable, and delivers precise results, making it an essential tool for biomedical research and drug development efforts.
Product Name:
Human HSP-90 (Heat Shock Protein 90) ELISA Kit
SKU:
HUES02816
Target:
Human HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-90. You can calculate the concentration of Human HSP-90 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
85-96
87-100
Average (%)
97
91
93
1:4
Range (%)
98-112
83-95
95-108
Average (%)
106
88
100
1:8
Range (%)
101-115
83-95
97-113
Average (%)
107
88
105
1:16
Range (%)
97-109
82-95
97-112
Average (%)
103
89
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
91-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.8
31.5
90.7
10.1
31.7
90.8
Standard deviation
0.5
1.6
3.4
0.7
1.7
4.2
C V (%)
5.1
5.08
3.75
6.93
5.36
4.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HSP-90 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HSP-90 in samples. No significant cross-reactivity or interference between Human HSP-90 and analogues was observed.
