Human hs-CRP (high-sensitivity C-reactive protein) CLIA Kit
The Human High Sensitivity C-Reactive Protein (hsCRP) CLIA Kit is a powerful tool for the precise measurement of hsCRP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.hsCRP is a key biomarker of inflammation and vascular disease, playing a crucial role in identifying and monitoring conditions such as cardiovascular diseases, autoimmune disorders, and infection.
By accurately quantifying hsCRP levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially develop targeted treatment strategies.Overall, the Human High Sensitivity C-Reactive Protein (hsCRP) CLIA Kit is an invaluable resource for researchers seeking to explore the role of hsCRP in health and disease, providing reliable data for advancing scientific understanding and clinical applications.
Product Name:
Human hs-CRP (high-sensitivity C-reactive protein) CLIA Kit
SKU:
HUES01286
Target:
Human hs-CRP (high-sensitivity C-reactive protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
94-106
86-100
Average (%)
98
101
92
1:4
Range (%)
99-113
102-119
96-108
Average (%)
106
109
102
1:8
Range (%)
94-111
99-114
96-112
Average (%)
102
106
104
1:16
Range (%)
91-101
88-98
88-100
Average (%)
96
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.0
68.98
181.94
29.6
71.22
177.57
Standard deviation
2.84
5.99
18.43
3.55
5.98
19.57
C V (%)
10.52
8.68
10.13
11.99
8.4
11.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human hs-CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human hs-CRP in samples. No significant cross-reactivity or interference between Human hs-CRP and analogues was observed.
