Human HPSE Recombinant Protein (RPPB1821)

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SKU:
RPPB1821
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
HPSE
Synonyms:
Heparanase
Synonyms:
HPA
Synonyms:
HSE1
Synonyms:
HPA1
Source:
Escherichia Coli
Uniprot:
Q9Y251
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Description

Product Name:Human HPSE Recombinant Protein
Product Code:RPPB1821
Size:20µg
Species:Human
Target:HPSE
Synonyms:Heparanase, HPA, HSE1, HPA1, HPR1, HPSE1, heparanase-1, EC 3.2.1.166, Endo-glucoronidase, HEP, Heparanase-1, Hpa1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:HPSE protein solution (1mg/ml) containing 20mM Tris-HCl (pH 8.0), 10% glycerol and 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greaterthan 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I

HPSEalso known as Heparanase-1 is an enzyme which cleaves heparan sulfateproteoglycans to allow cell movement through changing the extracellular matrix.In fact, the Heparan sulfate proteoglycans are major components of the basementmembrane and extracellular matrix. In addition, this cleavage can releasebioactive molecules from the extracellular matrix. HPSE is significant for theoverall degradation of proteins in lysosomes.

HPSE Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 531 amino acids (36-543 a.a) and having a molecular mass of 60kDa.HPSE is fused to a 23 amino acid His-tag at N-terminus.

UniProt Protein Function:HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.
UniProt Protein Details:

Protein type:EC 3.2.1.166; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; lysosomal membrane; lysosome; extracellular region; nucleus; lipid raft

Molecular Function:protein dimerization activity; syndecan binding; protein binding; beta-glucuronidase activity; heparanase activity

Biological Process: positive regulation of hair follicle development; proteoglycan metabolic process; positive regulation of protein kinase B signaling cascade; glycosaminoglycan catabolic process; heparan sulfate proteoglycan catabolic process; positive regulation of blood coagulation; glycosaminoglycan metabolic process; cell-matrix adhesion; positive regulation of osteoblast proliferation; carbohydrate metabolic process; pathogenesis; regulation of hair follicle development

NCBI Summary:Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q9Y251
NCBI GenInfo Identifier:296434532
NCBI Gene ID:10855
NCBI Accession:Q9Y251.2
UniProt Secondary Accession:Q9Y251,Q53GE5, Q9UL39, A9JIG7, C7F7I3, C7F7I4, E9PCA9 E9PGR1,
UniProt Related Accession:Q9Y251
Molecular Weight:42,791 Da
NCBI Full Name:Heparanase
NCBI Synonym Full Names:heparanase
NCBI Official Symbol:HPSE  
NCBI Official Synonym Symbols:HPA; HPA1; HPR1; HSE1; HPSE1  
NCBI Protein Information:heparanase; endo-glucoronidase; heparanase-1
UniProt Protein Name:Heparanase
UniProt Synonym Protein Names:Endo-glucoronidase; Heparanase-1; Hpa1
Protein Family:HpaII very short patch repair endonuclease
UniProt Gene Name:HPSE  
UniProt Entry Name:HPSE_HUMAN
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