The Human HPR (Haptoglobin-Related Protein) CLIA Kit is specifically designed for the precise measurement of haptoglobin-related protein levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research purposes.Haptoglobin-related protein is a vital protein that plays a crucial role in various physiological processes, including immune response modulation and binding to hemoglobin.
Dysregulation of this protein has been implicated in several diseases, making it a valuable biomarker for studying conditions like inflammation, cardiovascular diseases, and cancer.By using the Human HPR CLIA Kit, researchers can gain valuable insights into the role of haptoglobin-related protein in health and disease, facilitating the development of potential diagnostic tools and therapeutic strategies.
Product Name:
Human HPR (Haptoglobin Related Protein) CLIA Kit
SKU:
HUES00856
Target:
Human HPR (Haptoglobin Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HPR. You can calculate the concentration of Human HPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
97-113
84-98
Average (%)
94
105
91
1:4
Range (%)
101-113
87-102
90-102
Average (%)
108
93
95
1:8
Range (%)
98-115
100-116
92-106
Average (%)
105
107
99
1:16
Range (%)
95-107
96-109
100-117
Average (%)
102
103
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.51
4.29
0.48
1.66
4.07
Standard deviation
0.06
0.12
0.26
0.05
0.15
0.29
C V (%)
13.04
7.95
6.06
10.42
9.04
7.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HPR in samples. No significant cross-reactivity or interference between Human HPR and analogues was observed.
