The Human HP (Haptoglobin) CLIA Kit is specifically designed for the precise measurement of haptoglobin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Haptoglobin is a vital protein involved in binding free hemoglobin, preventing oxidative damage and inflammation. Abnormal haptoglobin levels have been linked to various health conditions, including hemolytic anemia, inflammation, and chronic kidney disease.
Therefore, reliable measurement of haptoglobin levels is essential for understanding and investigating these conditions, as well as potentially developing targeted therapies.Order the Human HP (Haptoglobin) CLIA Kit now to advance your research in areas such as hematology, inflammation, and kidney disease with confidence and precision.
Product Name:
Human HP (Haptoglobin) CLIA Kit
SKU:
HUES00855
Target:
Human HP (Haptoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HP. You can calculate the concentration of Human HP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
84-98
86-98
Average (%)
104
90
91
1:4
Range (%)
85-97
95-109
91-108
Average (%)
90
101
98
1:8
Range (%)
99-111
89-105
87-99
Average (%)
105
96
94
1:16
Range (%)
92-105
86-97
100-117
Average (%)
99
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-110
105
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.03
31.34
88.62
9.59
31.31
85.18
Standard deviation
1.15
2.79
6.66
1.15
3.24
7.15
C V (%)
11.47
8.9
7.52
11.99
10.35
8.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HP in samples. No significant cross-reactivity or interference between Human HP and analogues was observed.
