The Human HO-1 (Heme Oxygenase-1) ELISA Kit is a specialized assay designed for the precise measurement of HO-1 levels in human samples such as serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.HO-1 is a critical enzyme involved in the breakdown of heme and the maintenance of cellular homeostasis. Its dysregulation has been implicated in numerous health conditions, including oxidative stress-related diseases, inflammatory disorders, and various cancers.
Therefore, monitoring HO-1 levels can provide valuable insights into the pathogenesis of these diseases and facilitate the development of novel therapeutic strategies.Elevated HO-1 expression has also been associated with protective effects against tissue damage and inflammation, highlighting its potential as a biomarker for assessing disease severity and prognosis. With its advanced features and reliable performance, the Human HO-1 ELISA Kit is an indispensable tool for researchers seeking to delve deeper into the physiological and pathological roles of HO-1 in human health and disease.
Product Name:
Human HO1 (Heme Oxygenase 1) ELISA Kit
SKU:
HUES03073
Target:
Human HO1 (Heme Oxygenase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HO1. You can calculate the concentration of Human HO1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
99-111
88-103
Average (%)
95
105
94
1:4
Range (%)
94-106
80-91
89-100
Average (%)
100
86
95
1:8
Range (%)
88-100
85-101
86-97
Average (%)
94
92
92
1:16
Range (%)
90-106
86-97
80-94
Average (%)
97
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
92-110
100
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
3.15
9.57
0.99
3.28
9.54
Standard deviation
0.07
0.18
0.39
0.05
0.15
0.48
C V (%)
6.42
5.71
4.08
5.05
4.57
5.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HO1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed.
