Human HMGCR (3-Hydroxy-3-methylglutaryl CoA Reductase) ELISA Kit
The Human HMGCR (3-Hydroxy-3-methylglutaryl-coenzyme A reductase) ELISA Kit is specifically designed for the precise measurement of HMGCR levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.HMGCR is a key enzyme involved in the regulation of cholesterol synthesis and is known to play a critical role in lipid metabolism. Dysregulation of HMGCR has been linked to various metabolic disorders, including cardiovascular diseases and hypercholesterolemia.
Therefore, the detection of HMGCR levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human HMGCR ELISA Kit is a valuable tool for researchers looking to study the role of HMGCR in health and disease, offering reliable and reproducible results for enhanced scientific investigation.
Product Name:
Human HMGCR (3-Hydroxy-3-methylglutaryl CoA Reductase) ELISA Kit
SKU:
HUES03335
Target:
Human HMGCR (3-Hydroxy-3-methylglutaryl CoA Reductase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMG-CoAR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMG-CoAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMG-CoAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMG-CoAR. You can calculate the concentration of Human HMG-CoAR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
98-110
92-109
Average (%)
90
103
99
1:4
Range (%)
90-104
82-95
83-95
Average (%)
97
87
89
1:8
Range (%)
88-102
84-95
84-96
Average (%)
93
89
89
1:16
Range (%)
93-108
80-93
81-93
Average (%)
99
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.07
4.55
16.65
1.98
4.68
17.37
Standard deviation
0.13
0.23
0.54
0.12
0.19
0.76
C V (%)
6.28
5.05
3.24
6.06
4.06
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HMG-CoAR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HMG-CoAR in samples. No significant cross-reactivity or interference between Human HMG-CoAR and analogues was observed.
