Human HK3 Recombinant Protein (RPPB2549)
- SKU:
- RPPB2549
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- HK3
- Synonyms:
- Hexokinase-3
- EC 2711
- Hexokinase type III
- HK III
- Source:
- Escherichia Coli
- Uniprot:
- P52790
Description
| Product Name: | Human HK3 Recombinant Protein |
| Product Code: | RPPB2549 |
| Size: | 10µg |
| Species: | Human |
| Target: | HK3 |
| Synonyms: | Hexokinase-3, EC 2.7.1.1, Hexokinase type III, HK III, HXK3, HK3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The protein (1mg/ml) contains 20mM Tris pH 8.0 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDSIGSSGLR QGEETLSCSE EGLPGPSDSSE LVQECLQQFKVTRAQLQQI QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF LDAQPVNKQGLQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE CDVSLIPSVDGGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVAGVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS LALRQVRAIL EDLGLPLTSDDALMVLEVCQ AVSQRAAQLC GAGVAAVVEK IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE DGSGKGAALV TAVACRLAQL TRV |
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK3 encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.
HK-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain fused to His tag at the N-terminal encoding the sequence of 943 amino acids and having a molecular mass of 101.1 kDa.HXK3 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). |
| UniProt Protein Details: | Protein type:Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose Chromosomal Location of Human Ortholog: 5q35.2 Cellular Component: cytosol Molecular Function:fructokinase activity; glucokinase activity; hexokinase activity; mannokinase activity Biological Process: cell glucose homeostasis; glucose transport; glycolysis |
| NCBI Summary: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P52790 |
| NCBI GenInfo Identifier: | 206729871 |
| NCBI Gene ID: | 3101 |
| NCBI Accession: | P52790.2 |
| UniProt Secondary Accession: | P52790,Q8N1E7, |
| UniProt Related Accession: | P52790 |
| Molecular Weight: | 99,025 Da |
| NCBI Full Name: | Hexokinase-3 |
| NCBI Synonym Full Names: | hexokinase 3 |
| NCBI Official Symbol: | HK3 |
| NCBI Official Synonym Symbols: | HXK3; HKIII |
| NCBI Protein Information: | hexokinase-3 |
| UniProt Protein Name: | Hexokinase-3 |
| UniProt Synonym Protein Names: | Hexokinase type III; HK III |
| Protein Family: | Probable histidine kinase |
| UniProt Gene Name: | HK3 |
| UniProt Entry Name: | HXK3_HUMAN |
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