Human HIF-2 alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit
The Human HIF-2 alpha (Hypoxia-Inducible Factor 2 alpha) ELISA Kit is specifically designed for the quantitative detection of HIF-2 alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results essential for various research applications.HIF-2 alpha is a key transcription factor that plays a critical role in cellular response to hypoxia, regulating the expression of genes involved in angiogenesis, erythropoiesis, and metabolism. Dysregulation of HIF-2 alpha has been linked to a variety of diseases including cancer, cardiovascular disorders, and neurodegenerative conditions.
Therefore, the detection of HIF-2 alpha levels is crucial for understanding the underlying mechanisms of these diseases and identifying potential therapeutic targets.With its advanced technology and proven performance, the Human HIF-2 alpha ELISA Kit is a valuable tool for researchers looking to study the role of HIF-2 alpha in health and disease, ultimately leading to the development of innovative diagnostic and therapeutic strategies.
Product Name:
Human HIF-2 alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit
SKU:
HUES03331
Target:
Human HIF-2 alpha (Hypoxia Inducible Factor 2 Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-2α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-2α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-2α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HIF-2α. You can calculate the concentration of Human HIF-2α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
89-104
87-103
Average (%)
90
96
94
1:4
Range (%)
89-100
87-99
87-100
Average (%)
95
92
93
1:8
Range (%)
89-99
82-92
81-92
Average (%)
94
86
87
1:16
Range (%)
94-109
82-93
85-99
Average (%)
99
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.59
3.82
0.45
1.72
3.58
Standard deviation
0.02
0.08
0.2
0.02
0.1
0.16
C V (%)
4.17
5.03
5.24
4.44
5.81
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HIF-2α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HIF-2α in samples. No significant cross-reactivity or interference between Human HIF-2α and analogues was observed.
