The Human Hepc25 (Hepcidin-25) ELISA Kit is specifically designed for the precise measurement of hepcidin-25 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.Hepcidin-25 is a key regulator of iron metabolism, playing a crucial role in controlling iron absorption and distribution in the body. Dysregulation of hepcidin-25 levels has been linked to various disorders such as anemia, hemochromatosis, and inflammatory conditions, making it a vital biomarker for studying these diseases and developing potential treatments.
With the Human Hepc25 ELISA Kit, researchers can accurately assess hepcidin-25 levels in biological samples, helping to deepen our understanding of iron homeostasis and its impact on various health conditions. This kit provides a valuable tool for advancing research in the field of iron metabolism and related diseases.
Product Name:
Human Hepc25 (Hepcidin 25) ELISA Kit
SKU:
HUES03477
Target:
Human Hepc25 (Hepcidin 25)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Hepc25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Hepc25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Hepc25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Hepc25. You can calculate the concentration of Human Hepc25 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
92-106
92-104
Average (%)
98
98
97
1:4
Range (%)
92-107
86-97
83-97
Average (%)
98
92
88
1:8
Range (%)
92-104
86-97
89-101
Average (%)
99
91
95
1:16
Range (%)
90-100
82-95
88-98
Average (%)
95
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
92-109
100
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.77
12.6
39.73
5.22
12.14
40.95
Standard deviation
0.3
0.72
1.22
0.26
0.64
1.57
C V (%)
6.29
5.71
3.07
4.98
5.27
3.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Hepc25 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Hepc25 in samples. No significant cross-reactivity or interference between Human Hepc25 and analogues was observed.
